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| 期刊名称 |
| 年份 |
| 期卷号 |
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| DOI |
| 引用信息 |
| 作者 |
| 出版日期 |
| 作者单位 |
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| 期刊名称 | 年份 | 期卷号 | 标题 | DOI | 引用信息 | 作者 | 出版日期 | 作者单位 | 页码 | 摘要 |
|---|---|---|---|---|---|---|---|---|---|---|
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Environmental Kinetics of Radioactive Nuclides | https://doi.org/10.1269/jrr.42.436 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 436–438, | 2001/12/1 | "436-438" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Environmental and Ecological Effects of Environmental Pollutants | https://doi.org/10.1269/jrr.42.439 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 439–441, | 2001/12/1 | "439-441" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | p53 Function and Its Regulation Relevant to Adaptive Response to Radiation | https://doi.org/10.1269/jrr.42.442 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 442–443, | 2001/12/1 | "442-443" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Nuclear Fuel Cycle for 21 Century and Public Radioprotection | https://doi.org/10.1269/jrr.42.444 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 444–445, | 2001/12/1 | "444-445" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Front-runners in Study of Apoptosis: Mechanism and Significance in the Studies on Radiobiological Effects | https://doi.org/10.1269/jrr.42.446 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 446–447, | 2001/12/1 | "446-447" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Microbeam Irradiation: Studies on the Mechanism of Injuries to Cells and Trials for the Elucidation of Radiation-induced Adaptive Response | https://doi.org/10.1269/jrr.42.448 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 448–451, | 2001/12/1 | "448-451" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Radiation-induced Biological Response against Low Dose and Low Dose Rate of Irradiations | https://doi.org/10.1269/jrr.42.452 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 452–454, | 2001/12/1 | "452-454" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Radiation-induced Oxidative Injuries—Biological Significance and Mechanism of Protection | https://doi.org/10.1269/jrr.42.455 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 455–456, | 2001/12/1 | "455-456" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | New DNA Polymerases | https://doi.org/10.1269/jrr.42.457 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 457–458, | 2001/12/1 | "457-458" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Radiation-induced Genomic Instability | https://doi.org/10.1269/jrr.42.459 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 459–461, | 2001/12/1 | "459-461" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | DNA Double-Strand Breaks-Repair and Transmission of Signals | https://doi.org/10.1269/jrr.42.462 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 462–463, | 2001/12/1 | "462-463" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | JRRS and JSRC Joint Workshop: Research Collaboration System between Radiobiology and Radiochemistry | https://doi.org/10.1269/jrr.42.464 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 464–466, | 2001/12/1 | "464-466" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Radiation Epidemiology and Radiation Exposure | https://doi.org/10.1269/jrr.42.467 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 467–473, | 2001/12/1 | "467-473" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Environmental Effects of Radiations | https://doi.org/10.1269/jrr.42.474 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 474–478, | 2001/12/1 | "474-478" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Radiation Damage and Repair (I) | https://doi.org/10.1269/jrr.42.479 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 479–487, | 2001/12/1 | "479-487" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Radiation Damage and Repair (II) | https://doi.org/10.1269/jrr.42.488 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 488–497, | 2001/12/1 | "488-497" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Adaptive Response to Radiation (I) | https://doi.org/10.1269/jrr.42.498 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 498–506, | 2001/12/1 | "498-506" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Adaptive Response to Radiation (II) | https://doi.org/10.1269/jrr.42.507 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 507–514, | 2001/12/1 | "507-514" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Effects of Radiation (I) | https://doi.org/10.1269/jrr.42.515 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 515–523, | 2001/12/1 | "515-523" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Effects of Radiation (II) | https://doi.org/10.1269/jrr.42.524 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 524–533, | 2001/12/1 | "524-533" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Therapeutic Radiobiology | https://doi.org/10.1269/jrr.42.534 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 534–542, | 2001/12/1 | "534-542" | |||
| Journal of Radiation Research | 2001 | Volume 42, Issue 4, December | Non-ionizing Radiations and Radiation Physics | https://doi.org/10.1269/jrr.42.543 | Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 543–545, | 2001/12/1 | "543-545" | |||
| Journal of Radiation Research | 2002 | Volume 43, Issue 1, March | Pre-irradiation at a Low Dose-rate Blunted p53 Response | https://doi.org/10.1269/jrr.43.1 | Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 1–9, | Akihisa Takahashi* | 2002/3/1 | Akihisa Takahashi 1Department of Biology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634–8521, Japan Corresponding author: Phone; +81–744–29–8812, Fax; +81–744–25–3345, E-mail; atakahas@naramed-u.ac.jp | "1-9" | We investigated whethe chonic iadiation at a low dose-ate intefees with the p53-centeed signal tansduction pathway induced by adiation in human cultued cells and C57BL/6N mice. In in vito expeiments, we found that a challenge with X-ay iadiation immediately afte chonic iadiation esulted in lowe levels of p53 than those obseved afte the challenge alone in glioblastoma cells (A-172). In addition, the levels of p53-centeed apoptosis and its elated poteins afte the challenge wee stongly coelated with the above-mentioned phenomena in squamous cell cacinoma cells (SAS/neo). In in vivo expeiments, the accumulation of p53 and Bax, and the induction of apoptosis wee obseved dose-dependently in mouse spleen at 12 h afte a challenge with X-ays (3.0 Gy). Howeve, we found significant suppession of p53 and Bax accumulation and the induction of apoptosis 12 h afte challenge iadiation at 3.0 Gy with a high dose-ate following chonic pe-iadiation (1.5 Gy, 0.001 Gy/min). These findings suggest that chonic pe-iadiation suppessed the p53 function though adiation-induced signaling and/o p53 stability. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 1, March | Significance of the Response of Quiescent Cell Populations within Solid Tumors in Cancer Therapy | https://doi.org/10.1269/jrr.43.11 | Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 11–25, | Shin-Ichiro Masunaga*; Koji Ono | 2002/3/1 | Shin-Ichiro Masunaga 1Radiation Oncology Research Laboratory, Research Reactor Institute, Kyoto University, Noda, Kumatori-cho, Sennan-gun, Osaka 590–0494, Japan Corresponding author: Phone; +81–724–51–2406, 2487, 2475, Fax; +81–724–51–2627, E-mail; smasuna@rri.kyoto-u.ac.jp; Koji Ono 1Radiation Oncology Research Laboratory, Research Reactor Institute, Kyoto University, Noda, Kumatori-cho, Sennan-gun, Osaka 590–0494, Japan | "11-25" | In analyzing the esponse of quiescent (Q) cells in solid tumos, we have developed a combined method with a miconucleus (MN) assay and the identification of polifeating (P) cells by 5-bomo-2'-deoxyuidine (BdU) and an anti-BdU monoclonal antibody. Using this method, the esponses of Q tumo cells as well as total tumo (P+Q) cells within muine solid tumos to vaious DNA-damaging teatments wee evaluated. Based on this evaluation, combining with tiapazamine, a well-known bioeductive agent, and/o heat teatment at mild tempeatues was thought to be a pomising modality fo cance theapy in tems of conventional anticance teatment-esistant Q cell contol. Recently, ou method fo detecting the Q-cell esponse using P cell labeling with BdU and the MN fequency assay was also shown to be applicable to an apoptosis detection assay. Meanwhile, ou method fo detecting the intatumo Q-cell esponse was also applicable towad high linea enegy tansfe adiation, including eacto neutons. Thus, using ou method, a new neuton captue compound that has the potential to be distibuted in neuton captue theapy-esistant intatumo Q cell populations is now unde development. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 1, March | In Vitro Radiation-induced Effects on Rat Tracheal Epithelial CellsI) Different Radiosensitivity of Cell Inactivation after α and γ Irradiations | https://doi.org/10.1269/jrr.43.27 | Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 27–34, | Carole Kugel; Isabelle Bailly; Fran??oise Tourdes; Jean-Luc Poncy* | 2002/3/1 | Carole Kugel 1CEA/DSV/DRR/SRCA/Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France; Isabelle Bailly 1CEA/DSV/DRR/SRCA/Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France; Fran??oise Tourdes 1CEA/DSV/DRR/SRCA/Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France; Jean-Luc Poncy 1CEA/DSV/DRR/SRCA/Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France Corresponding author: Phone; (+) 33.1–69–26–63–91, Fax; (+) 33.1–69–26–70–45, E-mail; jean-luc.poncy@cea.fr | "27-34" | In ode to compae the adiotoxicity of alpha- and gamma-iadiations, pimay cultues of tacheal epithelial cells fom two at stains, Spague Dawley (SD) and Wista Futh/Fishe F344 (WF/Fi) ats, wee iadiated with 241Am α-paticles o 60Co γ-ays.The suvival atio fo each of the two at stain cells appeaed to be statistically diffeent afte high-LET iadiation. WF/Fi at cells wee 1.7-times moe adiosensitive than SD at cells, wheeas no diffeence was obseved following low-LET iadiation. A compaison of the cell suvival yielded RBEs of 2.8 and 4.5 fo SD and WF/Fi at cells, espectively. As peviously obseved, with inceasing LET of paticles, the cell-suvival cuves appoximate an exponential function of the dose. On the contay, fo low-LET, the suvival cuves showed a maked initial shoulde.This in vito cellula model, using epithelial cells of the uppe aiway, povides a suitable system to estimate the mechanism involved in adiosensitivity afte high-LET iadiation. The esponses to adiation-induced lethal effects within a same type of cell wee dependent on the iadiation paametes, but might be modulated by the individual sensitivity unde genetic o epigenetic facto contols. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 1, March | In Vitro Radiation-induced Effects on Rat Tracheal Epithelial Cells II) Different Preneoplastic Cell Transformation After α and γ Irradiations | https://doi.org/10.1269/jrr.43.35 | Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 35–42, | Jean-Luc Poncy*; Carole Kugel; Fran??oise Tourdes; Isabelle Bailly | 2002/3/1 | Jean-Luc Poncy 1CEA/DSV/DRR/SRCA/ Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France Corresponding author: Phone; (+) 33.1–69–26–63–91, Fax; (+) 33.1–69–26–70–45, E-mail: jean-luc.poncy@cea.fr; Carole Kugel 1CEA/DSV/DRR/SRCA/ Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France; Fran??oise Tourdes 1CEA/DSV/DRR/SRCA/ Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France; Isabelle Bailly 1CEA/DSV/DRR/SRCA/ Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France | "35-42" | In ode to compae the cell tansfomation induced by α- and γ-iadiation, pimay cultues of tacheal epithelial cells fom two at stains, Spague Dawley (SD) and Wista Futh / Fishe F344 (WF/Fi) ats, wee iadiated with 241Am α-paticles o 60Co γ-ays.The elative tansfomation fequency (RTF) fo WF/Fi pimay cells was vey close to the level of the spontaneous incidence and independent on the two iadiation types used. On the contay, the RTF fo the SD pimay cells inceased with a decease of the LET adiation when the elative suvival was highe than about 40%. Theefoe, the RTF values eached 4–5 fo α-paticles and 10–12 fo γ-ays.The RTF can be elated to the intinsic adiosensitivity of the at epithelial cells. Howeve, the diffeence in the adiation-induced RTF fo SD o WF/Fi pimay cells seems to be due to the development, unde genetic contol, of the initial lesion to the neoplastic state. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 1, March | A Nitroimidazole Derivative, PR-350, Enhances the Killing of Pancreatic Cancer Cells Exposed to High-dose Irradiation under Hypoxia | https://doi.org/10.1269/jrr.43.43 | Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 43–51, | Kazuhiro Mizumoto*; Li-Wu Qian; Li Zhang; Eishi Nagai; Shinobu Kura; Masao Tanaka | 2002/3/1 | Kazuhiro Mizumoto 1Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812–8582, Japan Corresponding author: Phone; +81–92–642–5444, Fax; +81–92–642–5458, E-mail; mizumoto@mailserver, www.med.kyushu-u.ac.jp/; Li-Wu Qian 1Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812–8582, Japan; Li Zhang 1Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812–8582, Japan; Eishi Nagai 1Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812–8582, Japan; Shinobu Kura 2Institute of Experimental Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812–8582, Japan; Masao Tanaka 1Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812–8582, Japan | "43-51" | The adiosensitizing effects of PR-350, a nitoimidazole deivative, wee examined concening the cell killing of human panceatic cance cell lines exposed to high doses of gamma-ay iadiation in vito. The pecentages of dead cells wee analyzed with a multiwell plate eade to measue the fluoescence intensity of popidium iodide befoe and afte a digitonin teatment. The sensitizing effect of PR-350 on cell killing by high-dose iadiation was confimed by time-couse, dose-dependency, and micoscopic obsevations. In five of seven panceatic cance cell lines in which the numbe of dead cells was detemined 5 days afte 30 Gy iadiation in the pesence of PR-350, the numbe was significantly inceased unde hypoxic conditions, but not unde aeobic conditions. The selective adiosensitive effect of PR-350 on hypoxic cells was also confimed by flow cytomety. The esults indicate that PR-350 can enhance the killing of panceatic cance cells by high-dose iadiation unde hypoxia, which suppots its clinical adiosensitizing effects when administeed duing intaopeative iadiation to panceatic cance. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 1, March | Reassessment of the Cancer Mortality Risk among Hiroshima Atomic-Bomb Survivors Using a New Dosimetry System, ABS2000D, Compared with ABS93D | https://doi.org/10.1269/jrr.43.53 | Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 53–63, | Hiroaki Katayama*; Masaaki Matsuura; Satoru Endo; Masaharu Hoshi; Megu Ohtaki; Norihiko Hayakawa | 2002/3/1 | Hiroaki Katayama 1Department of Information Technology, Radiation Effects Research Foundation, 5–2 Hijiyama-koen, Minami-ku, Hiroshima 732–0815, Japan Corresponding author: Phone; +81–82–261–1953, FAX; +81–82–261–4086, E-mail; H_katayama@rerf.jp; Masaaki Matsuura 2Department of Environmetrics and Biometrics, Division of Social Medicine, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1–2–3 Kasumi, Minami-ku, Hiroshima 734–8553, Japan; Satoru Endo 3Applied Physics and Chemistry, Graduate School of Engineering, Hiroshima University, 739–8527, Japan; Masaharu Hoshi 4International Radiation Information Center, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734–8553, Japan; Megu Ohtaki 2Department of Environmetrics and Biometrics, Division of Social Medicine, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1–2–3 Kasumi, Minami-ku, Hiroshima 734–8553, Japan; Norihiko Hayakawa 5Department of Epidemiology, Division of Social Medicine, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734–8553, Japan | "53-63" | The aim of the pesent study was to examine the excess elative isk fo leukemia motality and all cances, except leukemia, among Hioshima atomic-bomb suvivos by applying ABS93D and ABS2000D. Paticula attention was given to any diffeence in the neuton-dose estimates between the two dosimety systems. The study subjects wee 51,532 atomic-bomb suvivos egisteed in a database of the Reseach Institute fo Radiation Biology and Medicine of Hioshima Univesity (RIRBM). The esults obtained by both dosimety systems ae simila: the excess elative isk pe Sv fo leukemia motality and all cances except leukemia is significantly highe compaed to the contol goup. In addition, the diffeence in the excess elative isks between the two systems is not significant. Theefoe, it is indicated that a modification of the neuton-dose estimates would not makedly change the conclusions about the cance motality isk. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 1, March | The Quality of DNA Recovered from the Archival Tissues of Atomic Bomb Survivors is Good Enough for the Single Nucleotide Polymorphism Analysis in Spite of the Decade-long Preservation in Formalin | https://doi.org/10.1269/jrr.43.65 | Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 65–75, | Yumiko Nitta*; Hideo Tanaka; Yuji Masuda; Masaharu Hoshi | 2002/3/1 | Yumiko Nitta 1International Radiation Information Center, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi 1–2–3, Minami-Ku, Hiroshima 734–8553, Japan Corresponding author: Phone; 082–257–5875, Fax: 082–257–5877, E-mail; yumiko@hiroshima-u.ac.jp; Hideo Tanaka 2Department of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi 1–2–3, Minami-Ku, Hiroshima 734–8553, Japan; Yuji Masuda 3Department of Developmental Biology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi 1–2–3, Minami-Ku, Hiroshima 734–8553, Japan; Masaharu Hoshi 1International Radiation Information Center, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi 1–2–3, Minami-Ku, Hiroshima 734–8553, Japan | "65-75" | It is well known that the yield of DNA ecoveed fom tissues peseved in fomalin is invesely popotional to the stoed duation. How is the quality? We tested the quality of DNA fom achival tissues of atomic-bomb suvivos stoed in fomalin fo decades with the paametes of gene amplification efficiency by a polymease chain eaction. All of the DNA extacted fom the tissues peseved in fomalin fo 30 yeas amplified the 54-and 61-base pais of the DNA fagments successfully. The diect sequencing of the PCR poducts confimed the accuate amplification of the taget sequence. A futhe tial to amplify the longe sequence of 111 base pais succeeded in 20% of the samples tested. Fom these esults, we popose a new utility of achival samples fo the analysis of single nucleotide sequence polymophism of genes, no matte how long the samples have been peseved in fomalin. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 1, March | Radiation-chemical Properties of the Hypoxic Cell Radiosensitizer Doranidazole (PR-350) | https://doi.org/10.1269/jrr.43.77 | Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 77–88, | Mikinori Kuwabara*; Yoshiharu Iida; Osamu Inanami; Sadashi Sawamura; Kouji Yokoyama; Michihiko Tsujitani | 2002/3/1 | Mikinori Kuwabara 1Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan Corresponding author: Phone; +81–11–706–5235, Fax; +81–11–706–7373, E-mail; kuwabara@vetmed.hokudai.ac.jp; Yoshiharu Iida 1Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan; Osamu Inanami 1Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan; Sadashi Sawamura 2Laboratory of Applied Radiation Science, Graduate School of Engineering, Hokkaido University, Sapporo 060–8628, Japan; Kouji Yokoyama 3Pola Chemical Industries, Inc., Yokohama 221–0833, Japan; Michihiko Tsujitani 3Pola Chemical Industries, Inc., Yokohama 221–0833, Japan | "77-88" | This study was pefomed to confim the adiation-chemical popeties of the 2-nitoimidazole deivative doanidazole, (±)-(2RS,3SR)-3-(2-nitoimdazol-1-yl)-methoxybutane-1,2,4-tiol CAS 137339–64–1, PR-350, which was synthesized as a hypoxic cell adiosensitize with low toxicity. Radiation-chemical expeiments using doanidazole showed that (1) unlike O2, it had high eactivity towad not only hydated electons (eaq-), but also hydoxyl adicals (·OH), (2) the educed intemediates of doanidasole had no ability to induce immediate stand beaks of colE1 plasmid DNA, (3) doanidazole enhanced adiation-induced DNA stand beaks of colE1 plasmid DNA in the aqueous state, wheeas it did not enhance the base alteation, such as 8-oxo-deoxyguanosine, (4) it enhanced the adiation-induced fomation of stand beaks with 3'-phosophate and 3'- phosphoglycolate temini, and (5) it was bound to DNA afte iadiation. These facts evealed that the majoity of adiation-chemical popeties of doanidazole, except fo the high eactivity towad ·OH, wee simila to those of oxygen. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 1, March | Types and Three-dimensional Distribution of Neuronal Ectopias in the Brain of Mice Prenatally Subjected to X-irradiation | https://doi.org/10.1269/jrr.43.89 | Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 89–98, | Xue-Zhi Sun*; Sentaro Takahashi; Yoshihisa Kubota; Hiroshi Sato; Chun Cui; Yoshihiro Fukui; Minoru Inouye | 2002/3/1 | Xue-Zhi Sun 1Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences, 4–9–1, Inage-ku, Chiba 263–8555, Japan Corresponding author: Phone; +81–43–206–3159, Fax; +81–43–251–4853, E-mail; sun_s@nirs.go.jp; Sentaro Takahashi 1Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences, 4–9–1, Inage-ku, Chiba 263–8555, Japan; Yoshihisa Kubota 1Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences, 4–9–1, Inage-ku, Chiba 263–8555, Japan; Hiroshi Sato 1Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences, 4–9–1, Inage-ku, Chiba 263–8555, Japan; Chun Cui 2Department of Anatomy, School of Medicine, Tokushima University, 3–18–15 Kuramoto-cho, Tokushima 770–8503, Japan; Yoshihiro Fukui 2Department of Anatomy, School of Medicine, Tokushima University, 3–18–15 Kuramoto-cho, Tokushima 770–8503, Japan; Minoru Inouye 3Shin Nippon Biomedical Laborarories, Ltd. 2438 Miyanoura, Yoshida, Kagoshima 891–1394, Japan | "89-98" | The types and thee-dimensional distibution of neocotical ectopias following penatal exposue to X-iadiation wee studied by a histological examination and compute econstuction techniques. Pegnant ICR mice wee subjected to X-iadiation at a dose of 1.5 Gy on embyonic day 13. The bains fom 30-day-old mice wee seially sectioned on the fontal plane at 15 μm, stained with HE and obseved with a micoscope. The image data fo the sections wee input to a compute, and then econstucted to thee-dimensional bain stuctues using the Magellan 3.6 pogam. Sectional images wee then dawn on a compute display at 240 μm intevals, and the positions of the diffeent types of neocotical ectopias wee maked using colo coding. Thee types of neocotical ectopias wee ecognized in the iadiated bains. Neocotical Lay I ectopias wee identified as small patches in the caudal occipital cotex, and wee located moe lateally in the neocotex in caudal sections than in the ostal sections. Peiventicula ectopias wee located moe ostally than Lay I ectopias, and wee found fom the most caudal extent of the pesumed moto cotex to the most caudal extent of the lateal venticle. Hippocampal ectopias appeaed as continuous linea bands, and wee fequently associated with the anteio pats of the peiventicula ectopias. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 1, March | Redox Reactions of Tocopherol Monoglucoside in Aqueous Solutions: A Pulse Radiolysis Study | https://doi.org/10.1269/jrr.43.99 | Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 99–106, | Sudhir Kapoor*; Tulsi Mukherjee; Tsutomu V. Kagiya; Cherupally Krishnan K. Nair | 2002/3/1 | Sudhir Kapoor 1Radiation Chemistry Chemical Dynamics Division, Bhabha Atomic Research Centre, Mumbai 400 085, India Corresponding author: Phone; 91–22–5505291, FAX: 91–22–5505151, E-mail; sudhirk@apsara.barc.ernet.in; Tulsi Mukherjee 1Radiation Chemistry Chemical Dynamics Division, Bhabha Atomic Research Centre, Mumbai 400 085, India; Tsutomu V. Kagiya 2Health Research Foundation, Kyoto 606–8225, Japan; Cherupally Krishnan K. Nair 3Radiation Biology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India | "99-106" | The eactions between tocopheol monoglucoside (TMG), a wate-soluble vitamin-E deivative, with B2· -, N3·, (SCN)2·-, NO2·, OH· and vaious halogenated peoxyl adicals wee examined using a pulse adiolysis technique. The esults demonstate that TMG foms a stable phenoxyl adical at pH > 6.8. The thus-fomed phenoxyl adical shows pH-dependent decay kinetics and is dispopotionated by 2nd ode kinetics at pH 2.3. It was obseved that the TMG eactivity towads a halogenated peoxyl adical inceases with the numbe of halogen atoms at the cabon atom having a peoxyl goup. The eaction between the TMG phenoxyl adical and ascobic acid was also examined using a pulse adiolysis technique. The esults indicate that the TMG phenoxyl adical is epaied by ascobate. Kinetic studies indicate that TMG may act as an antioxidant to epai fee-adical damage to some biologically impotant compounds. The one-electon eduction potential fo TMG was found to be 0.522 V ± 0.06 vs. NHE. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 2, June | Cytogenetic Changes in the Liver of Progeny of Irradiated Male Rats | https://doi.org/10.1269/jrr.43.125 | Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 125–133, | Katarína Kropá??ová; Lucia Slovinská*; Eva Mi??úrová | 2002/6/1 | Katarína Kropá??ová 1Department of Cellular and Molecular Biology, Faculty of Sciences, P. J. ??afárik University, Moyzesova 11, 04167 Ko??ice, Slovakia; Lucia Slovinská 1Department of Cellular and Molecular Biology, Faculty of Sciences, P. J. ??afárik University, Moyzesova 11, 04167 Ko??ice, Slovakia Corresponding author: Phone: 04215562 22610, Fax: 04215562 22124, E-mail: slovins@kosice.upjs.sk; Eva Mi??úrová 1Department of Cellular and Molecular Biology, Faculty of Sciences, P. J. ??afárik University, Moyzesova 11, 04167 Ko??ice, Slovakia | "125-133" | The tansgeneational tansmission of adiation damage of at genom was studied on the basis of cytogenetic changes in somatic cells (hepatocytes). It was found, that the iadiation of at males with dose of 3 Gy of gamma adiation caused latent cytogenetic damage to the live, which was expessed duing the couse of an induced polifeation of hepatocytes (by patial hepatectomy) by lowe polifeative activity and a high fequency of chomosomal abeations. In the pogeny of iadiated males (in the F1 geneation), the adiation damage to DNA was manifested by simila changes, i.e. by lowe polifeation activity and incease in “spontaneus” chomosomal abeation occuence in live egeneation afte patial hepatectomy. Iadiating the pogeny of iadiated males (the total adiation load of the pogeny being 3 Gy + 3 Gy) caused slighte changes in compaed with iadiating the pogeny of non-iadiated contol males (the total adiation load of the pogeny being 0 Gy + 3 Gy), which suggests some kind of adaptive esponse, which was also found in othe expeimental systems and paametes. An analogous couse of RNA and DNA quantitative changes in the live of the F0 and F1 geneations of ats confims the patial tansmission of adiation damage of genom to the pogeny. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 2, June | Establishment of an Animal Model for Radiation-induced Vomiting in Rats Using Pica | https://doi.org/10.1269/jrr.43.135 | Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 135–141, | Kouichi Yamamoto; Noriaki Takeda; Atsushi Yamatodani* | 2002/6/1 | Kouichi Yamamoto 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7, Yamadaoka, Suita, Osaka 565–0871, Japan; Noriaki Takeda 2Department of Otorhinolaryngology, School of Medicine, University of Tokushima, 3–18–15, Kuramoto-cho, Tokushima 770–8503, Japan; Atsushi Yamatodani 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7, Yamadaoka, Suita, Osaka 565–0871, Japan Corresponding author: Phone: +81–6–6879–2562, Fax: +81–6–6879–2562, E-mail: yamatoda@sahs.med.osaka-u.ac.jp | "135-141" | We investigated whethe adiation-induced pica, a behavio chaacteized by the eating of a non-food substance, such as kaolin, can be used as an index of adiation-induced vomiting in ats. Since thee was an individual diffeence in the susceptibility to pica, we selected ats that actually ate kaolin following X-ay iadiation, and used them fo the expeiment. The total-body iadiation (TBI) inceased kaolin consumption in a dose-dependent manne (sham, 0.05 ± 0.03 (SEM) g; 2 Gy, 0.38 ± 0.11 g; 4 Gy, 1.54 ± 0.28 g; 8 Gy, 3.55 ± 0.67 g), and the inceased kaolin consumption afte 4 Gy of TBI was inhibited by a peteatment with the seotonin 5-HT3 ecepto antagonist ondanseton (2 mg/kg, i.p.) (saline, 1.49 ± 0.33 g; ondanseton, 0.75 ± 0.11 g). Futhemoe, 4 Gy of abdominal iadiation was moe effective to induce pica than that of head iadiation (abdomen: 0.37 ± 0.05 g, head: 0.06 ± 0.01 g). These findings suggested that peipheal seotonegic pathway is pedominantly involved in the development of adiation-induced pica in ats and that the adiation-induced pica could be useful as a behavioal index fo the seveity of adiation-induced vomiting in ats. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 2, June | Histological and Elemental Changes in the Rat Brain after Local Irradiation with Carbon Ion Beams | https://doi.org/10.1269/jrr.43.143 | Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 143–152, | Sentaro Takahashi*; Xue-Zhi Sun; Yoshihisa Kubota; Nobuhiko Takai; Kumie Nojima | 2002/6/1 | Sentaro Takahashi 1Environmental and Toxicological Research Group, National Institute of Radiological Sciences Anagawa 4–9–1, Inage-ku, Chiba-shi, Chiba 263–8555, Japan Corresponding author: Phone: +81–43–206–3158, Fax: +81–43–251–4853, E-mail: sentaro@nirs.go.jp; Xue-Zhi Sun 1Environmental and Toxicological Research Group, National Institute of Radiological Sciences Anagawa 4–9–1, Inage-ku, Chiba-shi, Chiba 263–8555, Japan; Yoshihisa Kubota 1Environmental and Toxicological Research Group, National Institute of Radiological Sciences Anagawa 4–9–1, Inage-ku, Chiba-shi, Chiba 263–8555, Japan; Nobuhiko Takai 2Research Laboratory of Space Radiation, National Institute of Radiological Sciences Anagawa 4–9–1, Inage-ku, Chiba-shi, Chiba 263–8555, Japan; Kumie Nojima 2Research Laboratory of Space Radiation, National Institute of Radiological Sciences Anagawa 4–9–1, Inage-ku, Chiba-shi, Chiba 263–8555, Japan | "143-152" | The left ceebal hemisphees of adult Spague-Dawley at bains wee iadiated at doses of 30, 50, o 100 Gy with chaged cabon paticles (290 MeV/nucleon; 5 mm spead-out Bagg peak). The spead-out Bagg peak used hee successfully and satisfactoily etained its high-dose localization in the defined egion. A histological examination showed that necotic tissue damage, hemohage in the thalamus, and vasodilatations aound the necotic egion wee induced at 8 weeks afte 100 Gy iadiation. The egions with tissue damage coelated well with those expected fom the adiation-dose distibution, indicating an advantage of chaged cabon paticles fo iadiating esticted bain egions. An X-ay fluoescent analysis demonstated a decease in the concentations of K and P, and an incease in the concentations of Cl, Fe, Zn in the damaged egion at 8 weeks post-iadiation, though no significant changes wee obseved befoe 4 weeks of post-iadiation. This may indicate that even the vey high adiation doses used hee did not induce acute and immediate neuonal cell death, in contast with ischemic bain injuy whee acute neuonal cell death occued and the elemental concentations changed within a day afte the induction of ischemia. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 2, June | Inhibition of γ-Radiation Induced DNA Damage in Plasmid pBR322 by TMG, a Water-soluble Derivative of Vitamin E | https://doi.org/10.1269/jrr.43.153 | Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 153–159, | Rema Rajagopalan; Khalida Wani; Nagaraj G. Huilgol; Tsutomu V. Kagiya; Cherupally K. Krishnan Nair* | 2002/6/1 | Rema Rajagopalan 1Radiation Biology Division, Bhabha Atomic Research Centre, Mumbai 400 085; Khalida Wani 2Department of Radiation Oncology, Nanavati hospital MRC, Vile Parle, India; Nagaraj G. Huilgol 2Department of Radiation Oncology, Nanavati hospital MRC, Vile Parle, India; Tsutomu V. Kagiya 3Kinki Research Foundation, Kyoto, Japan; Cherupally K. Krishnan Nair 1Radiation Biology Division, Bhabha Atomic Research Centre, Mumbai 400 085 Corresponding author: Phone: 91–22–5593869, Fax: 91–22–5560750/91–22–5505151, E-mail: cknair@magnum.barc.ernet.in | "153-159" | Alpha-tocopheol monoglucoside (TMG), a wate-soluble deivative of α-tocopheol, has been examined fo its ability to potect DNA against adiation-induced stand beaks. Gamma adiation, up to a dose of 6 Gy (dose ate, 0.7 Gy/ minute), induced a dose-dependent incease in single stand beaks (SSBs) in plasmid pBR322 DNA. TMG inhibited the fomation of γ-adiation induced DNA single stand beaks (SSBs) in a concentation-dependent manne; 500 μM of TMG potected the single stand beaks completely. It also potected thymine glycol fomation induced by γ-adiation in a dose-dependent manne, based on an estimation of thymine glycol by HPLC. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 2, June | Effect of a Hypoxic Cell Sensitizer Doranidazole on the Radiation-induced Apoptosis of Mouse L5178Y Lymphoma Cells | https://doi.org/10.1269/jrr.43.161 | Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 161–166, | Mizuho Aoki*; Yoshiya Furusawa; Yuta Shibamoto; Ataru Kobayashi; Michihiko Tsujitani | 2002/6/1 | Mizuho Aoki 1Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan Corresponding author: Phone: +81–43–206–3232, Fax: +81–43–206–4149, E-mail: mizuho@nirs.go.jp; Yoshiya Furusawa 2Department of Therapeutic Radiology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoinkawara-machi, Sakyo-ku, Kyoto 606–8507, Japan; Yuta Shibamoto 2Department of Therapeutic Radiology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoinkawara-machi, Sakyo-ku, Kyoto 606–8507, Japan; Ataru Kobayashi 3Pharmaceuticals Division, Pola Chemical Industries, Inc., 560 Kashiocho Totsuka-ku, Yokohama, Kanagawa 244–0814, Japan; Michihiko Tsujitani 4Pola Chemical Industries, Inc., 27–1 Takashimadai, Kanagawa-ku, Yokohama 221–0833, Japan | "161-166" | We investigated the sensitizing effect of the 2-nitoimidazole analogue doanidazole, a new hypoxic adiosensitize, on adiation-induced apoptosis in L5178Y cells. Apoptosis was assessed by checking DNA ladde fomation, the pesence of sub-G1 peaks in flow cytomety, and chomatin condensation. A adiosensitizing effect of doanidazole was also confimed by a soft-aga colony assay of suviving cells. In the assay of DNA ladde fomation, DNA fagmentation was obseved following iadiation unde an aeobic o hypoxic condition with o without doanidazole. The popotions of the cells at the sub-G1 peak in a flow cytometic measuement was not vey diffeent among the iadiations at 5 Gy unde the aeobic condition, 15 Gy unde hypoxia, and 10 Gy with 1 mM doanidazole unde hypoxia. The faction of cells with chomatin condensation was found to be significantly inceased with doanidazole up to 3 mM when applied unde hypoxic iadiation, but did not incease even at 10 mM. The sensitize enhancement atio was estimated to be about 1.7 with a concentation of 1 mM. This enhancement atio was not diffeent fom that obseved by assaying cell suvivals. On the othe hand, doanidazole showed no adiosensitizing effect unde aeobic conditions with 1 mM. In conclusion, the adiation-induced apoptosis of L5178Y cells was enhanced by doanidazole unde hypoxia. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 2, June | Establishment of Immortal Normal and Ataxia Telangiectasia Fibroblast Cell Lines by Introduction of the hTERT Gene | https://doi.org/10.1269/jrr.43.167 | Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 167–174, | Hideaki Nakamura*; Hiroko Fukami; Yuko Hayashi; Tohru Kiyono; Shigekazu Nakatsugawa; Michinari Hamaguchi; Kanji Ishizaki | 2002/6/1 | Hideaki Nakamura 1Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute, Kanokoden 1–1, Chikusa-ku, Nagoya 464–8681, Japan2Department of Molecular Pathogenesis, Nagoya University School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya 466–8550, Japan Corresponding author: Phone: +81–52–762–6111 (ext. 7012), Fax: +81–52–763–5233, E-mail: hnakamu@aichi-cc.jp; Hiroko Fukami 1Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute, Kanokoden 1–1, Chikusa-ku, Nagoya 464–8681, Japan; Yuko Hayashi 1Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute, Kanokoden 1–1, Chikusa-ku, Nagoya 464–8681, Japan; Tohru Kiyono 3Division of Virology, Aichi Cancer Center Research Institute, Kanokoden 1–1, Chikusa-ku, Nagoya 464–8681, Japan; Shigekazu Nakatsugawa 4Department of Radiology, Nagoya University School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya 466–8550, Japan; Michinari Hamaguchi 2Department of Molecular Pathogenesis, Nagoya University School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya 466–8550, Japan; Kanji Ishizaki 1Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute, Kanokoden 1–1, Chikusa-ku, Nagoya 464–8681, Japan | "167-174" | To establish immotal human cells, we intoduced the human catalytic subunit of telomease (hTERT) gene into skin fiboblast cells obtained fom nomal and ataxia telangiectasia (AT) individuals of Japanese oigin. Afte hTERT intoduction, these cells continue to gow beyond a population doubling numbe of 200 while maintaining thei oiginal adiosensitivity. Inductions of p53, phosphoylation of Se15 in p53, and induction of p21 by X-ay iadiation in immotal cells deived fom nomal individual wee not affected by the hTERT intoduction. Both nomal and AT immotal cells exhibited an appaent inhibition of gowth as oiginal pimay cells when they eached confluence. Kayotype analysis has evealed that they ae in a diploid ange. These esults suggest that cells immotalized by hTERT intoduction etain thei oiginal chaacteistics except fo immotalization, and that they may be useful fo analyzing vaious effects of adiation on human cells. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 2, June | Putative Tumor-suppressor Gene Regions Responsible for Radiation Lymphomagenesis in F1 Mice with Different p53 Status | https://doi.org/10.1269/jrr.43.175 | Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 175–185, | Doo-Pyo Hong; Nobuko Mori; Seiichi Umesako; Chang-Woo Song; Yeong-Gwan Park; Shiro Aizawa; Masaaki Okumoto* | 2002/6/1 | Doo-Pyo Hong 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Nobuko Mori 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan; Seiichi Umesako 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Chang-Woo Song 3Korea Research Institute of Chemical Technology, P.O.Box 107, Yusong, Taejon 305–606, Korea; Yeong-Gwan Park 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Shiro Aizawa 4Division of Biology and Oncology, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Masaaki Okumoto 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan Corresponding author: Phone: +81 722 54 9837, Fax: +81 722 54 9837, E-mail: okumoto@riast.osakafu-u.ac.jp | "175-185" | Regions of allelic loss on chomosomes in many tumos of human and some expeimental animals ae geneally consideed to habo tumo-suppesso genes involved in tumoigenesis. Allelotype analyses have geatly impoved ou undestanding of the molecula mechanism of adiation lymphomagenesis. Peviously, we and othes found fequent loss of heteozygosity (LOH) on chomosomes 4, 11, 12, 16 and 19 in adiation-induced lymphomas fom seveal F1 hybid mice. To examine possible contibutions of individual tumo-suppesso genes to tumoigenesis in p53 heteozygous deficiency, we investigated the genome-wide distibution and status of LOH in adiation-induced lymphomas fom F1 mice with diffeent p53 status. In this study, we found fequent LOH (moe than 20%) on chomosomes 4 and 12 and on chomosomes 11, 12, 16 and 19 in adiation-induced lymphomas fom (STS/A X MSM/Ms)F1 mice and (STS/A X MSM/Ms)F 1-p53KO/+ mice, espectively. Low incidences of LOH (10–20%) wee also obseved on chomosomes 11 in mice with wild-type p53, and chomosomes 1, 2, 9, 17 and X in p53 heteozygous-deficient mice. The fequency of LOH on chomosomes 9 and 11 inceased in the (STS/A X MSM/Ms)F1- p53KO/+ mice. Pefeential losses of the STS-deived allele on chomosome 9 and wild-type p53 allele on chomosome 11 wee also found in the p53 heteozygous-deficient mice. Thus, the putative tumo-suppesso gene egions esponsible fo lymphomaganesis might consideably diffe due to the p53 status. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 2, June | Generation of Large Homozygous Chromosomal Segments by Mitotic Recombination during Lymphomagenesis in F1 Hybrid Mice | https://doi.org/10.1269/jrr.43.187 | Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 187–194, | Doo-Pyo Hong; Kihei Kubo; Naomi Tsugawa; Nobuko Mori; Seiichi Umesako; Chang-Woo Song; Masaaki Okumoto* | 2002/6/1 | Doo-Pyo Hong 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Kihei Kubo 2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Naomi Tsugawa 2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Nobuko Mori 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan; Seiichi Umesako 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Chang-Woo Song 3Korea Research Institute of Chemical Technology, P.O.Box 107, Yusong, Taejon 305–606, Korea; Masaaki Okumoto 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan Corresponding author: Phone: +81 722 54 9837, Fax: +81 722 54 9837, E-mail: okumoto@riast.osakafu-u.ac.jp | "187-194" | The loss of heteozygosity (LOH) has been epoted in numeous neoplasms in both human and animals, and has often been obseved in chomosomal egions, which contain tumo-suppesso genes. We peviously found fequent LOH on chomosomes 4, 12 and 19 in adiation-induced lymphomas fom (BALB/cHeA x STS/A)F1 hybid mice by allelotype analysis at polymophic micosatellite loci. In this study, to elucidate the natue of allelic losses, we efined the loss egions on chomosomes 4, 12 and 19 of the tumos fom the F1 mice and then analyzed them cytogenetically. The esults epesent evidence of a wide ange of allelic losses owing to mitotic ecombination on chomosomes 4 and 19 in the tumos, possibly eflecting functional losses of putative tumo-suppesso genes. It is suggested that the geneation of these lage homozygous chomosomal segments pobably containing the affected genes is one of the genetic alteations esponsible fo tumoigenesis. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 2, June | Characterization of Spontaneous Mutation in the ΔsoxR and SoxS Overproducing Strains of Escherichia coli | https://doi.org/10.1269/jrr.43.195 | Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 195–203, | Eiji Yamamura; Eun Hye Lee; Akihiro Kuzumaki; Norio Uematsu; Tatsuo Nunoshiba; Masakado Kawata; Kazuo Yamamoto* | 2002/6/1 | Eiji Yamamura 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan2Mitsubishi Pharma Corporation, Chiba 292–0812, Japan; Eun Hye Lee 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan3Present Address; Department of Aquatic Life Medicine, Pukyong National University, Pusan 608–737, Korea; Akihiro Kuzumaki 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan; Norio Uematsu 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan; Tatsuo Nunoshiba 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan; Masakado Kawata 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan; Kazuo Yamamoto 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan Corresponding author: Phone: +81–22–217–5054, Fax: +81–22–217–5053, E-mail: yamamot@mail.cc.tohoku.ac.jp | "195-203" | To examine the ole of the soxRS egulon in mutagenesis, we chaacteized the spontaneous mutations occuing in the endogenous tonB gene in the ΔsoxR stain and the SoxS ovepoducing stain of Escheichia coli. Neithe the ΔsoxR stain no the SoxS ovepoducing stain led to an enhancement o diminishment of the spontaneous mutation fequency. By DNA sequencing, we detemined 50 spontaneous mutants fom the ΔsoxR stains, and found that 36% wee both base substitutions and IS insetions, 14% fameshifts and 10% deletions. Among the base substitutions, G:C→T:A tansvesions and G:C→A:T tansitions pedominated, followed by A:T→T:A tansvesions. We detemined 54 spontaneous mutants fom the SoxS ovepoducing stains, and found that 37% wee IS insetions, 31% base substitutions, 17% fameshifts, 9% deletions and 6% duplications. Among the base substitutions, G:C→T:A tansvesions dominated, followed by A:T →T:A tansvesions and G:C→A:T tansitions. These esults wee simila to those fom the soxRS+ stains. Thus, it is suggested that the soxRS-egulated genes do not play a significant ole in the defense against spontaneous mutagenesis. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 2, June | Whole-body X-irradiation Induces Acute and Transient Expression of Heme Oxygenase-1 in Rat Liver | https://doi.org/10.1269/jrr.43.205 | Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 205–210, | Keiko Suzuki*; Masahiko Mori; Fumihiko Kugawa; Hiroshi Ishihara | 2002/6/1 | Keiko Suzuki 1Redox Regulation Research Group, National Institute of Radiological Sciences, Anagawa 4–9–1, Inage-ku, Chiba 263–8555, Japan Corresponding author: Phone: +81–43–206–3127, Fax: +81–43–255–6819, E-mail: suzuki_q@nirs.go.jp; Masahiko Mori 2Radiation Hazard Research Group, National Institute of Radiological Sciences, Anagawa 4–9–1, Inage-ku, Chiba 263–8555, Japan; Fumihiko Kugawa 3Department of Biological Pharmaceutical Sciences, College of Pharmacy, Nihon Univ., 7–7–1 Narashino-dai, Funabashi 274–8555, Japan; Hiroshi Ishihara 1Redox Regulation Research Group, National Institute of Radiological Sciences, Anagawa 4–9–1, Inage-ku, Chiba 263–8555, Japan | "205-210" | Activation of the stess-inducible heme oxygenase-1 (HO-1) gene by X-iadiation was investigated in at live. When male Wista MS stain ats (8 weeks) eceived whole-body iadiation of 17.0 Gy, 7 h late the activity of heme oxygenase in the live was significantly enhanced (2.5 times). The level of HO-1 mRNA expession was inceased by 2.3 and 4.0 times 2 and 4 h afte adiation, and then declined at 7 and 10 h to the level of 2.0 and 1.6 times of the contol. When the X-ay dose was vaied fom 4.0 to 21.7 Gy, the tansciption of the gene was enhanced at all doses and the level of activation was dose-dependent. Finally, westen blotting of iadiated live demonstated a significant incease in the level of HO-1 induced by X-ays, peaking at 4 h. Thus, X-ays wee confimed to be stessos that induce acute HO-1 expession tansiently in the live. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 3, September | Dimerization, Translocation and Localization of Ku70 and Ku80 Proteins | https://doi.org/10.1269/jrr.43.223 | Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 223–236, | Manabu Koike* | 2002/9/1 | Manabu Koike 1Radiation Hazards Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan Corresponding author: Phone: 81–43–206–3139, Fax: 81–43–206–3139, E-mail: m_koike@nirs.go.jp | "223-236" | The Ku potein is a complex of two subunits, Ku70 and Ku80, and was oiginally identified as an autoantigen ecognized by the sea of patients with autoimmune diseases. The Ku potein plays a key ole in multiple nuclea pocesses, e.g., DNA epai, chomosome maintenance, tansciption egulation, and V(D)J ecombination. The mechanism undelying the egulation of all the divese functions of Ku is still unclea, although it seems that Ku is a multifunctional potein that woks in nuclei. On the othe hand, seveal studies have epoted cytoplasmic o cell suface localization of Ku in vaious cell types. To claify the fundamental chaacteistics of Ku, we have examined the expession, heteodimeization, subcellula localization, chomosome location, and molecula mechanisms of the nuclea tanspot of Ku70 and Ku80. The mechanism that egulates fo nuclea localization of Ku70 and Ku80 appeas to play, at least in pat, a key ole in egulating the physiological function of Ku in vivo. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 3, September | Drinking Beer Reduces Radiation-induced Chromosome Aberrations in Human Lymphocytes | https://doi.org/10.1269/jrr.43.237 | Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 237–245, | Manami Monobe; Koichi Ando* | 2002/9/1 | Manami Monobe 1Graduate School of Science and Technology, Chiba University, 1–33, Yayoi-cho, Inage-ku, Chiba 263–8522, Japan2Division of Heavy-ion Radiobiology Research Group, National Institute of Radiological Sciences, 9–1, Anagawa-4-chome, Inage-ku, Chiba 263–8555, Japan; Koichi Ando 2Division of Heavy-ion Radiobiology Research Group, National Institute of Radiological Sciences, 9–1, Anagawa-4-chome, Inage-ku, Chiba 263–8555, Japan Corresponding author: Phone: +81–43–206–3231, Fax: +81–43–206–4149, E-mail: ando@nirs.go.jp | "237-245" | We hee investigated and epoted the effects of bee dinking on adiation-induced chomosome abeations in blood lymphocytes. Human blood that was collected eithe befoe o afte dinking a 700 ml bee was in vito iadiated with 200 kVp X ays o 50 keV/μm cabon ions. The elation between the adiation dose and the abeation fequencies (fagments and dicentics) was significantly (p ?? 0.05) lowe fo lymphocytes collected 3 h afte bee dinking than those befoe dinking. Fitting the dose esponse to a linea quadatic model showed that the alpha tem of cabon ions was significantly (p ?? 0.05) deceased by bee dinking. A decease of dicentic fomation was detected as ealy as 0.5 h afte bee dinking, and lasted not shote than 4.5 h. The mitotic index of lymphocytes was highe afte bee dinking than befoe, indicating that a division delay would not be esponsible fo the low abeations induced by bee dinking. An in vito teatment of nomal lymphocytes with 0.1 M ethanol, which coesponded to a concentation of 6-times highe than the maximum ethanol concentation in the blood afte bee dinking, educed the dicentic fomation caused by X-ay iadiation, but not by cabon-ion iadiation. The bee-induced eduction of dicentic fomation was not affected by seum. It is concluded that bee could contain non-ethanol elements that educe the chomosome damage of lymphocytes induced by high-LET adiation. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 3, September | Relative Biological Effectiveness of 290 MeV/u Carbon Ions for the Growth Delay of a Radioresistant Murine Fibrosarcoma | https://doi.org/10.1269/jrr.43.247 | Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 247–255, | Sachiko Koike*; Koichi Ando; Chisa Oohira; Takeshi Fukawa; Ryonfa Lee; Nobuhiko Takai; Manami Monobe; Yoshiya Furusawa; Mizuho Aoki; Shigeru Yamada; Wakako Shimizu; Kumie Nojima; Hideyuki Majima | 2002/9/1 | Sachiko Koike 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan Corresponding author: Phone: +81–43–251–2111 ext. 6921, Fax: +81–43–206–4149, E-mail: koike@nirs.go.jp; Koichi Ando 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Chisa Oohira 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Takeshi Fukawa 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Ryonfa Lee 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Nobuhiko Takai 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Manami Monobe 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Yoshiya Furusawa 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Mizuho Aoki 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Shigeru Yamada 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Wakako Shimizu 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Kumie Nojima 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Hideyuki Majima 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan | "247-255" | The elative biological effectiveness (RBE) fo animal tumos teated with factionated doses of 290 MeV/u cabon ions was studied. The gowth delay of NFSa fibosacoma in mice was investigated following vaious daily doses given with cabon ions o those given with cesium γ-ays, and the RBE was detemined. Animal tumos wee iadiated with cabon ions of vaious LET (linea enegy tansfe) in a 6-cm SOBP (spead-out Bagg peak), and the isoeffect doses; i.e. the dose necessay to induce a tumo gowth delay of 15 days wee studied. The isoeffect dose fo cabon ions of 14 and 20 keV/μm inceased with an incease in the numbe of factions up to 4 factions. The incease in the isoeffect dose with the faction numbe was small fo cabon ions of 44 keV/μm, and was not obseved fo 74 keV/μm. The α and β values of the linea-quadatic model fo the adiation dose-cell suvival elationship wee calculated by the Fe-plot analysis method. The α values inceased linealy with an incease in the LET, while the β values wee independent of the LET. The α/β atio was 129 ± 10 Gy fo γ-ays, and inceased with an incease in the LET, eaching 475 ± 168 Gy fo 74 keV/μm cabon ions. The RBE fo cabon ions elative to Cs-137 γ-ays inceased with the LET. The RBE values fo 14 and 20 keV/μm cabon ions wee 1.4 and independent of the numbe of factions, while those fo 44 and 74 keV/μm inceased fom 1.8 to 2.3 and fom 2.4 to 3.0, espectively, when the numbe of factions inceased fom 1 to 4. Inceasing the numbe of factions futhe fom 4 to 6 was not associated with an incease in the RBE. These esults togethe with ou ealie study on the skin eaction suppot the use of an RBE of 3.0 in clinical tials of 80 keV/μm cabon beams. The RBE values fo low doses of cabon beams wee also consideed. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 3, September | Models for Mixed Irradiation with a ‘Reciprocal-Time’ Pattern of the Repair Function | https://doi.org/10.1269/jrr.43.257 | Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 257–267, | Shozo Suzuki*; Yuri Miura; Shoichi Mizuno; Yoshiya Furusawa | 2002/9/1 | Shozo Suzuki 1Redox Regulation Research Group, Tokyo Metropolitan Institute of Gerontology, Chiba 263–8555, Japan Corresponding author: Phone:+81–3–3964–3241 ext. 3142, Fax: +81–3–3579–4776, E-mail: shozo@tmig.or.jp; Yuri Miura 1Redox Regulation Research Group, Tokyo Metropolitan Institute of Gerontology, Chiba 263–8555, Japan; Shoichi Mizuno 1Redox Regulation Research Group, Tokyo Metropolitan Institute of Gerontology, Chiba 263–8555, Japan; Yoshiya Furusawa 1Redox Regulation Research Group, Tokyo Metropolitan Institute of Gerontology, Chiba 263–8555, Japan | "257-267" | Suzuki pesented models fo mixed iadiation with two and multiple types of adiation by extending the Zaide and Rossi model, which is based on the theoy of dual adiation action. In these models, the epai function was simply assumed to be semi-logaithmically linea (i.e., monoexponential), o a fist-ode pocess, which has been expeimentally contadicted. Fowle, howeve, suggested that the epai of adiation damage might be lagely a second-ode pocess athe than a fist-ode one, and pesented data in suppot of this hypothesis. In addition, a second-ode epai function is pefeed to an n-exponential epai function fo the eason that only one paamete is used in the fome instead of 2n-1 paametes fo the latte, although both epai functions show a good fit to the expeimental data. Howeve, accoding to a second-ode epai function, the epai ate depends on the dose, which is incompatible with the expeimental data. We, theefoe, evised the models fo mixed iadiation by Zaide and Rossi and by Suzuki, by substituting a ‘ecipocal-time’ patten of the epai function, which is deived fom the assumption that the epai ate is independent of the dose in a second-ode epai function, fo a fist-ode one in eduction and inteaction factos of the models, although the undelying mechanism fo this assumption cannot be well-explained. The eduction facto, which educes the contibution of the squae of a dose to cell killing in the linea-quadatic model and its deivatives, and the inteaction facto, which also educes the contibution of the inteaction of two o moe doses of diffeent types of adiation, wee fomulated by using a ‘ecipocal-time’ patten of the epai function. Cell suvivals calculated fom the olde and the newly modified models wee compaed in tems of the dose-ate by assuming vaious types of single and mixed iadiation. The esult implies that the newly modified models fo mixed iadiation can expess o pedict cell suvival moe accuately than the olde ones, especially when iadiation is polonged at low dose ates. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 3, September | Effects of Heavy-ion Radiosurgery on the Hemopoietic Function of the Silkworm Bombyx mori | https://doi.org/10.1269/jrr.43.269 | Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 269–275, | Zhen-Li Tu*; Yasuhiko Kobayashi; Kenji Kiguchi; Hiroshi Watanabe; Kazuo Yamamoto | 2002/9/1 | Zhen-Li Tu 1Biotechnology Laboratory, Department of Radiation Research for Environment and Resources, Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute (JAERI), Watanukimachi 1233, Takasaki, Gunma 370–1292, Japan Corresponding author: Phone: 81–027–346–9542, Fax: 81–027–346–9688, E-mail: tu514@yahoo.co.jp; Yasuhiko Kobayashi 1Biotechnology Laboratory, Department of Radiation Research for Environment and Resources, Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute (JAERI), Watanukimachi 1233, Takasaki, Gunma 370–1292, Japan; Kenji Kiguchi 1Biotechnology Laboratory, Department of Radiation Research for Environment and Resources, Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute (JAERI), Watanukimachi 1233, Takasaki, Gunma 370–1292, Japan; Hiroshi Watanabe 1Biotechnology Laboratory, Department of Radiation Research for Environment and Resources, Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute (JAERI), Watanukimachi 1233, Takasaki, Gunma 370–1292, Japan; Kazuo Yamamoto 1Biotechnology Laboratory, Department of Radiation Research for Environment and Resources, Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute (JAERI), Watanukimachi 1233, Takasaki, Gunma 370–1292, Japan | "269-275" | To study the effects of heavy-ion adiosugey on the hemopoietic function of a silkwom, hemopoietic ogans of lavae wee locally iadiated with cabon-ion beams, and the changes in the hemocyte density and in the hemocyte function wee investigated. When the lavae wee iadiated by 50 Gy to 300 Gy cabon ions on the 3d day of the 4th insta, the hemocyte densities did not change fo a while, though they gadually inceased at a late stage, but wee finally still significantly lowe than those of uniadiated contols. The hemocyte densities of the lavae iadiated at diffeent developmental stages showed suppessed incements, and cabon-ion iadiation given to lavae at ealy stages compaed to the late stages had a significant suppessive effect on the hemocyte densities. On unilateal iadiated lavae a hemocyte intemediate incement between those of bilateal iadiated lavae and uniadiated contols was obseved. The pecentage of dead hemocytes was obviously highe fo iadiated lavae than uniadiated contols duing the late 5th insta. Thus, it is evident that cabon-ion adiosugey on hemopoietic ogans of silkwom induced not only a quantitative change, but also a qualitative change in the hemocytes. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 3, September | Changes in Histological Construction and Decrease in 3H-QNB Binding in the Rat Brain after Prenatal X-irradiation | https://doi.org/10.1269/jrr.43.277 | Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 277–282, | Sotaro Momosaki; Xue-Zhi Sun; Nobuhiko Takai; Rie Hosoi; Osamu Inoue; Sentaro Takahashi* | 2002/9/1 | Sotaro Momosaki 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7 Yamada-oka, Suita, Osaka 565–0871, Japan; Xue-Zhi Sun 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7 Yamada-oka, Suita, Osaka 565–0871, Japan; Nobuhiko Takai 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7 Yamada-oka, Suita, Osaka 565–0871, Japan; Rie Hosoi 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7 Yamada-oka, Suita, Osaka 565–0871, Japan; Osamu Inoue 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7 Yamada-oka, Suita, Osaka 565–0871, Japan; Sentaro Takahashi 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7 Yamada-oka, Suita, Osaka 565–0871, Japan Corresponding author: Phone: 043–206–3158, Fax: 043–251–4853, E-mail: sentaro@nirs.go.jp | "277-282" | To elucidate the mechanisms involved in deleteious neuonal and behavioal changes afte penatal ionizing iadiation, in vito muscainic acetylcholine (mACh) ecepto binding and histological constuction wee investigated in 9-week old at bains afte 1.5 Gy X-ay exposue on embyonic day 15 (E15). A goss anatomical examination with a magnetic-esonance imaging system showed an iegula tissue constuction in the hippocampus and cotex of the iadiated at bain. Histological sections stained with hematoxylin and eosin also indicated that the stuctues of the hippocampus and cotex wee obviously changed. In iadiated ats, the lamina stuctue of pyamidal cells was selectively deanged in the CA1 egion. In vito3H-Quinuclidinyl benzilate binding in the hippocampus was significantly deceased (about 10%) in penatal iadiated ats compaed to that in sham-teated ats. On the othe hand, no significant change in mACh ecepto binding was obseved in the ceebal cotex. The pesent study evealed that penatal exposue to ionizing adiation may induce dysfunction of the cholinegic neuonal systems, especially in the hippocampus, esulting in deleteious changes in memoy and behavio. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 3, September | Radiosensitization by Overexpression of the Nonphosphorylation Form of IκB-α in Human Glioma Cells | https://doi.org/10.1269/jrr.43.283 | Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 283–292, | Naoko Honda; Kasumi Yagi; Gui-Rong Ding; Junji Miyakoshi* | 2002/9/1 | Naoko Honda 1Department Radiation Genetics, Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-Ku, Kyoto 606–8501, Japan; Kasumi Yagi 1Department Radiation Genetics, Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-Ku, Kyoto 606–8501, Japan2Department of Zoology, Graduate School of Science, Kyoto University, Yoshida-Konoe-cho, Sakyo-Ku, Kyoto 606–8501, Japan; Gui-Rong Ding 1Department Radiation Genetics, Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-Ku, Kyoto 606–8501, Japan3Department of Radiation Medicine, Fourth Military Medical University, Xi'an 710032, China; Junji Miyakoshi 1Department Radiation Genetics, Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-Ku, Kyoto 606–8501, Japan Corresponding author: Phone; +81–75–753–4666, Fax; +81–75–753–4669, E-mail, miyakosh@mfour.med.kyoto-u.ac.jp | "283-292" | To assess the ole of NF-κB in cellula adiosensitivity, we constucted mutated IκB expession plasmids fo SY-IκB (with mutations at esidues of 32, 36 and 42) expession in human malignant glioma cells (adiosensitive MO54 and adioesistant T98 cells), giving espective cell types efeed to as MO54-SY4 and T98-SY14. Both of the clones expessing SY-I κB became adiosensitive, compaed with the paental MO54 and T98 cells. A teatment with hebimycin A o genistein did not change the adiosensitivity of cells expessing SY-IκB, but made both the MO54 and T98 paental cells moe sensitive to ionizing adiation. A teatment with TNF-α induced DNA fagmentation and apoptosis in cells expessing SY-IκB, but not in MO54 and T98 cells. The suvival afte X-ay exposue of the paental MO54 cells was slightly inceased by a TNF-α teatment, but that of the paental T98 cells did not change. The change in sensitivity to ulta-violet (UV) adiation and adiamycin in MO54-SY4 cells was vey simila to that fo X-ay sensitivity, but no change was obseved in T98-SY14 cells. Significant sublethal damage epai was obseved in T98 cells, wheeas MO54 cells showed little epai activity. The expession of p53 was enhanced in the paental MO54 cells, while the p53 levels in the MO54-SY4, and in the paent and clonal T98 cells, did not change. Ou data suggest that the seine and tyosine phosphoylation of IκB-α may play a ole in detemining the adiosensitivity of malignant glioma cells. |
| Journal of Radiation Research | 2002 | Volume 43, Issue 3, September | Radioprotective Effects of 2-Imino-3-(chromone-2-yl)carbonyl thiazolidines against γ-Irradiation in Mice | https://doi.org/10.1269/jrr.43.293 | Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 293–300, | Seyed Jalal Hosseinimehr; Abbas Shafiee*; Hossein Mozdarani; Shahram Akhlagpour; Mohsen Froughizadeh | 2002/9/1 | Seyed Jalal Hosseinimehr 1Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Abbas Shafiee 1Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Corresponding author: Tel.: +98–21–6406757; Fax: +98–21–6461178; E-mail: ashafiee@ams.ac.ir; Hossein Mozdarani 1Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Shahram Akhlagpour 1Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Mohsen Froughizadeh 1Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran | "293-300" | A seies of 2-imino-3-(chomone-2-yl) cabonylthiazolidines substituted at the C-5 and / o C-7 positions of a chomone ing wee synthesized. The in vivo toxicity and adiopotective efficacy of these agents wee evaluated in male NMRI mice against cobalt-60 γ-ays. The LD50 values as detemined by a Pobit analysis, wee 659, 1216 and 790 mg/kg fo compounds, 2, 3 and 4, espectively. Fo studying adiopotective effects, one half of the toxic LD50 values wee used, namely 330, 605 and 395 mg/kg fo compounds 2, 3 and 4, espectively. The dose educed facto (DRF) was detemined by dividing the LD50/30 values obtained fom the adiation suvival cuve in the pesence of a adiopotective agent by the LD50/30 value obtained fom a contol adiation suvival cuve. A compound with a hydoxyl goup substituent at the C-5 position affoded bette adiopotective activity than those without this substituent. The adiopotective effect of chomone having a hydoxyl goup at only the C-7 position was simila to that of the unsubstituted chomone. The most active compound has hydoxyl goups at the C-5 and C-7 positions of the chomone ing; it had a DRF of 1.48. |
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